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Liquid Chromatography Home > Analytical Chemistry > Liquid Chromatography LIQUID CHROMATOGRAPHY Basic Liquid Chromatography Advantages of Liquid Chromatography (LC) vs. Gas Chromatography (GC) Gradient Elution in Liquid Chromatography Identifying Compounds by LC/HPLC - Qualitative Analysis by LC / HPLC Ion-Exchange Chromatography (IEC) - Liquid Chromatography Separation Modes     Liquid Chromatography (LC): Columns in LC/HPLC – Bonded Phase Columns (C18) Liquid Chromatography / HPLC Separation Modes: Steric Exclusion Chromatograply (SEC) Liquid Chromatography: Column Theory, Chromatographic Resolution, Column Efficiency     Normal phase liquid chromatography (LC) / HPLC? Selection & Optimization of operating parameters in Liquid Chromatography (LC) Selectio...

In a previous post entitled  " TroubleshootingLC / HPLC Systems – Tailing Peaks " peak tailing due to bad columns and sample overload was explained and methods were proposed to fix it.  Peaks with strange shapes represent one of the most common problems that can arise in the LC/HPLC laboratory.  It should be first explained why tailing is bad : Peaks with tailing can be hard to integrate The precision and the reliability of assay methods involving distorted – peaks with tailing – is often poor when compared to good chromatography Resolution is low when peaks show tailing  Another cause of peak tailing is when wrong solvent is used to dissolve the sample. Wrong Solvent for Sample Peak tailing is observed when the volume and the kind of solvent used to dissolve the sample is not appropriate. Ideally a small volume of sample (dissolved in the mobile phase) should be injected. However, when a relatively large volume of a solvent stronger than the mobile phase is injec...